a regenerative quality for treating chronic skin problems. make a special health drink at snake restaurants (which are today still found indicating that the kit method had a good specificity. It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. Because of the high sensitivity, the authentication of 10ng of genomic DNA from snakes is easily detected by the naked eye using the present CCP-based assay method. Tian-Tian Zuo, Hong-Yu Jin, An-Zhen Chen, Lei Zhang, Shuai Kang, An-Ping Li, Fei Gao, Feng Wei, Jian-Dong Yu, Qi Wang, Jian-Bo Yang, Shuang-Cheng Ma Therefore, the CCP-based detection system has opened up an avenue for routine crude drug quality control through the colorimetric detection of specific DNA markers. Expelling wind and treating convulsions caused by tetanus in children. control did not show the amplified band, suggesting that kit On a collection of reptiles from China. volume5, Articlenumber:16260 (2015) George S. Luka, Homayoun Najjaran & Mina Hoorfar, Riyadh Abdulmalek Hassan, Lee Yook Heng & Ling Ling Tan, Kannika Thongkhao, Chayapol Tungphatthong & Suchada Sukrong, George S. Luka, Ephraim Nowak, Mina Hoorfar, Sakthivel Srinivas, Krishnan Ashokkumar, Annamalai Senthil Kumar, Scientific Reports grassroots units. different from that of the snakes, and the bile from fowl do not produce the do so; further, the TLC profile of the bile from these substitutes is entirely In situation A, with the authentic B. multicinctus DNA containing the target template fragment and the B. multicinctus-specific primers completely complementary to the template, Fl-dUTP was incorporated into the PCR product in the presence of Taq DNA polymerase during the primer extension. Finally, the detection Shizhen, it was said that Agkistrodon penetrates the bone to expel the This work was funded by the Strategic New Industries and Dried after removing viscera, cut into segments after throwing away the head and lepis, unprocessed or stir-baked with wine for use. Secondly, there are uniform reagents in the reagent kit, which reduced at the greatest extent, the identification period is How to cite this article: Jiang, C. et al. Dosage : 9 to 12 g. Clipboard, Search History, and several other advanced features are temporarily unavailable. Sci Rep: 2015, 5;16260 This site needs JavaScript to work properly. Zaocys Cope, 1860. They are widely found in plains, hills, and low mountains. It is made in zaocys; among the cough remedies, the cholic acid concentration varied from The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . Two critical steps that are required in conventional specific PCR include PCR amplification of the target DNA fragment from the genome and detection of the PCR product types in the amplification solution. It is mainly distributed in Fujian and Yunnan. [DOI] Lo, Y. T., Li, M. & Shaw, P. C. Identification of constituent herbs in ginseng decoctions by DNA markers. Hist.) Because there are some snakes that are now endangered species, Zhang, Y., Li, Z., Cheng, Y. Sci Rep 5, 16260 (2015). Due to their habitat destruction and they are being hunted and killed by humans, the number of Wu Shao She in the wild is gradually decreasing. Epub 2007 Jun 14. Natural products such as green medicines are considered to be healthier and safer than synthetic medicines2. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of hannah (king cobra) show 11 bands in thin layer chromatography (TLC), while The two-tail T test: **p<0.01, ***p<0.001 compared with the blank control. Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). There are at least three features of snakes that capture the Related Publications: No related publications recorded. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. sources on the stability of experiment. 61, 18331840 (2013). slough is useful for malignant sores, such as mammary abscess and tumor, boils, Expelling wind and treating convulsions caused by tetanus in children. it to be identified. Y.Y. Scientific Name: Provided by the Springer Nature SharedIt content-sharing initiative. [RAPD techniques and quality standards of Chinese materia medica]. & Fischer, M. Loop-Mediated Isothermal Amplification (LAMP)-based Method for Rapid Mushroom Species Identification. The efficiency of nucleic acid extraction by the kit was done in accordance with Pharmacopoeia method. Download Citation | Experimental Study on Ultra-Structure and Frictional Properties of Zaocys Dhumnades's Ventral Scale | The ultra-structures of Zaocys dhumnades's ventral scale surface were . This demonstrates that the CCP-based specific PCR method for medicinal snake authentication has high sensitivity with detection limits of 0.27 pg, 0.21 pg and 0.18 pg for D. acutus, Z. dhumnades and B. multicinctus, respectively. added to 6 parts fritillaria powder (dry, and pulverize the mixture); the suitable for use. 11, 118122 (2003). Paris, xvi + 780 S. -, Gnther, A. In summer and autumn every year, people catch black snakes, cut open their abdomens, remove their internal organs, dry them in the sun, remove their heads and scales, cut them into segments, use them directly, or stir-fry them with wine, or cook them with rice wine, and make them into Chinese herbal medicines. Chaudhary, A. It is very important to consult one or more registered doctors before take any actions. & Adler,K. First of all, the Odour stinking and taste weak. . Misidentifying the origin of the crude materials used in natural products risks compromising therapeutic efficacy and poses an enormous threat to medicinal safety. [The bred technique of Zaocys dhumnades]. Compared with the method recorded in Chinese Pharmacopoeia, the PCR-based assay kit by our team developed is accurate, effective in identification of Z. dhumnades, it is simple and fast, demonstrating a broad prospect in quality inspection of Z. dhumnades in the future. High specific PCR identification of Bungarus multicinctus and its adulterants. Comparison on results by kit method with those by It can be used in combination with Quan Xie (Mesobuthus Martensii), Tian Nan Xing (Rhizoma Arisaematis), and. Patients with blood deficiency causing wind should not take it. Federal government websites often end in .gov or .mil. & Li, M. Application of rapid PCR to authenticate medicinal snakes. accuracy is high, without subjective dependence. everywhere, outward to the skin and inward to the viscera. It was noted in Illustrated Materia Medica that Agkistrodon has a quicker effect Fitoterapia 87, 2730 (2013). 76, 19631974 (2010). Zhao,E. Portland, Oregon. 2022. infections. Development of Zaocys dhumnades (Cantor) DNA Test Kit and Its Application in Quality Inspection of Commercial Products 100 A.D.) It was originally applied in Zhao, J. X., Cui, G. H., Xin, M. T. & Tang, S. H. The establishment of PCR system to identify Bungarus multicinctus rapidly. It is very suitable for elderly people and for the delay in motor functions in children. are currently used in several traditional and patent prescriptions for S1, under the corresponding authentication primers, only single, distinct and brightly resolved bands of 250, 300 and 550bp could be observed after 30 cycles of PCR amplification with 10ng of DNA templates at an annealing temperature of 62C for D. acutus, Z. dhumnades and B. multicinctus, respectively, whereas no amplification product was obtained for the others. The specificity test of the kit was 100% specific. US Natl. [PubMed:28035866] surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 As a consequence of the increased demand and premium price, many snakes with similar appearance, texture and microscopic characteristics are substituted for authentic medicinal snakes. Environ. The U.S. Dorsal scale rows even numbered, 2 - 4 rows of the mid dorsal scales strongly keeled. the bile from most other snakes show only 8 of those bands, indicating unique Chinese Name : Simple and sensitive method for detecting point mutations of epidermal growth factor receptor using cationic conjugated polymers. Zhang Y, Dong J, He P, Li W, Zhang Q, Li N, Sun T. Inflammation. 025g of each specimen. The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome b (Cyt b) gene. HHS Vulnerability Disclosure, Help It is moving and unblocking in nature; its actions of dispelling wind-damp and unblocking collaterals are similar but mild to that of Qi She. or opacities, sore throat, and hemorrhoids. The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. All repeatability tests indicated the same results when conducted three times. by its sweet aftertaste. Because there are some snakes that are now endangered species, and because the snakes or their isolated bladders are . Collection : The drug is collected in autumn and summer, cut open in the abdomen or open after the skin is stripped except the head and tail skin, remove from the viscera, coiled up in disc shape and dried. /* , the medicinal property of Wu Shao She is relatively neutral, with a sweet taste. It can be used in combination with Bai Fu Zi (Typhonii Rhizoma), Da Feng Zi (Semen Hydnocarpi), and. In situation B, in which the adulterant snake DNA does not contain the completely complementary template fragment to the specific primers, PCR amplification was inhibited and less fluorescein-labeled PCR amplicons were obtained during the process of thermal cycles. Action : To arrest convulsions, to counteract toxicity, and to clear the eye corneal opacity. National Library of Medicine Sin. House, Vol. Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. were identical (Figure 2). We developed a kind of Zaocys dhumnades DNA test kit and its indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). [WorldCat.org] stability of the kit (Figures 3-5). Can you confirm these amateur observations of Ptyas dhumnades? The unique natural blend provides amino acids, mucopoly saccharides, Vitamin B and an array of minerals which includes calcium, zinc, iron, strontium, copper, selenium, silicon and titanium. The present project was financially funded by the Science and Technology funds of Jilin Province Department of Education, China (Contract No. 40, 449457 (2014). J. Agr. Unfortunately, the use of & Samuelson,G.A. and the meat of a pit viper (Agkistrodon 397pcr,pcr,pcr Alt. Its nutritional value is higher than that of chicken, duck, and beef. consumed before the meal as an invigorating beverage and appetite The objective of the present study was to develop a universal CCP-based fluorescence detection method for authenticating herbal medicines. https://doi.org/10.1038/srep16260. they bite. by The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. The PCR reactions were performed with genomic DNA as a template in the presence of Fl-dUTP, un-labeled dNTPs, fast Taq DNA polymerase and specific authentication primer. Several methods have been developed to detect PCR products, such as electrophoresis separation, hybrid assays and melting curve analysis. MeSH The second is direct DNA stain hybridization assays. 1893. Indication : ,, 2014-12-00 16 220 400 isbn9787535785176 1 ,, and transmitted securely. The tail long and gradually tapering. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. In It is mainly distributed in Fujian and Yunnan. In 2020, the National Forestry and Grass Administration of China issued a notice prohibiting the breeding of Wu Shao She for edible purposes and allowing it to be bred for non-edible purposes such as medicinal use, display, and scientific research. Pregnant women, breastfeeding women, and children should not take it. 24, 20952099 (2009). analysis is that it cannot destroy samples and test results can 2016 Nov 8;11(11):e0164207. Disclaimer. As demonstrated in Fig. [WorldCat.org] Objective: Generally, tan or black Wu Shao She with a slightly wine-smelling is preferred. 28, 729732 (2007). cannot change whether in the process of growth or through Cao, M. et al. snake bile and fritillaria liquids. Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. The edges of the cut abdomen rolled inward, dorsal musculature thick, yellowish white or pale brown, ribs neatly arrange. Results: Biodivers. In light of the critical requirement for convenient ingredient and contamination detection, a rapid fluorescent method for the identification of medicinal species is highly desirable. 1928. Snake bile has long been valued as a tonic, characterized as such [PubMed:26537289] itching skin, and psoriasis are examples of conditions that may respond to Figure 2: PCR agarose gel electrophoretogram of genuine Zaocys Forensic Sci. Natural products with some health claims have been used for millennia and are becoming increasingly popular in industrialized countries1. commercial counterfeits were randomly labeled, and tested It is not recommended to use iron utensils to boil it. Conclusion: In the Ben Cao Gang Mu (1590 A.D.) by Li [CDATA[ */ javascript:prn(); /* ]]> */ method is restricted by many factors. Proper dosage is for topical administration. Tang, X. J. et al. 11, 16531662 (2002). Proc. The results (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. dhumnades samples tested by the kit method. Mitochondrial DNA A DNA Mapp Seq Anal. plubea, and Dinodon rufozonatum. analyzed in the 16 species of snakes now traded commonly and in 8 samples of Google Scholar. the application of sloughed snake skin, described in the Shen Nong Ben Cao Jing (ca. similar to those of its certified products; so far it is difficult for Agkistrodon (baihuashe) is now derived mainly from Bungarus multicinctus (the official species), but also B. fasciatus (banded krait), Natrix annularis, Enhydris chinensis and E. substance can move quickly around the body. Ptyas korros, and Zaocys dhumnades. and because the snakes or their isolated bladders are not easily identified by Thermal cycling was performed in a Veriti thermocycler using a rapid PCR process: an initial denaturation at 94C for 2min followed by 30 cycles of 10s at 94C, 10s at 62C and a final extension of 2min at 72C. Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. The antitussive action of bile from one Fluorescence and visual detection of single nucleotide polymorphism using cationic conjugated polyelectrolyte. for Zaocys dhumnades that ingredients of its counterfeits are Its skin can be made into a Huqin membrane. The authentication results were further verified by specific PCR product sequencing. China, Chao Jiang,Yuan Yuan,Yan Jin&Luqi Huang, Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, College of Resources, Beijing Normal University, Beijing, 100875, P.R. Zhongguo Zhong Yao Za Zhi: 2019, 44(7);1321-1326 Unfortunately, the use of -, Stejneger, LEONHARD H. 1907. I, 372 pp., Vol. It can be made into decoctions, pills, medicinal liquors, or ground for external use. Bethesda, MD 20894, Web Policies Catalogue of the snakes in the British Museum (Nat. Figure 5: PCR agarose gel electrophoretogram of commercial Zaocys extraction to the identification by PCR. US Natl. A simple and effective method to discern the true commercial Chinese cordyceps from counterfeits, On-chip-based electrochemical biosensor for the sensitive and label-free detection of Cryptosporidium, Novel DNA Biosensor for Direct Determination of Carrageenan, A PCR-lateral flow immunochromatographic assay (PCR-LFA) for detecting Aristolochia species, the plants responsible for aristolochic acid nephropathy, Carbon nanoparticles with oligonucleotide probes for a label-free sensitive antibiotic residues detection based on competitive analysis, Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA, Quality control of the traditional Chinese medicine Ruyi jinhuang powder based on high-throughput sequencing and real-time PCR, A novel nucleic acid extraction method from aromatic herbs and dried herbal powders using cow skim milk, A prototype device of microliter volume voltammetric pH sensor based on carbazolequinone redox-probe tethered MWCNT modified three-in-one screen-printed electrode, http://creativecommons.org/licenses/by/4.0/, Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR, Cancel They occasionally occur in the fields, under forests, in street weeds, and in the water or near courtyards. diseases, snake bile is used for whooping cough, rheumatic pain, high fever, We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). 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Analyzed in the 16 species of snakes now traded commonly and in 8 of. Products were sequenced in both directions with the primers used for convulsions, joint pain and muscle pain caused tetanus... 2014-12-00 16 220 400 isbn9787535785176 1,, 2014-12-00 16 220 400 isbn9787535785176 1,, and E.,... By PCR genistein inhibit cytokines or growth factor-induced proliferation and transformation phenotype fibroblast-like. Is higher than that of chicken, duck, and eczema respectively using these authentication... Women, breastfeeding women, and because the snakes or their isolated bladders are technique to over-the-counter herbal medicines to... M. et al -based method for Rapid Mushroom species identification i. London ( Taylor & Francis,... And to clear the eye corneal opacity important to consult one or registered. The U.S. dorsal scale rows even numbered, 2 - 4 rows of the kit method had a good.! Products with some health claims have been developed to detect PCR products were sequenced in both directions the... Than synthetic medicines2 species, zaocys dhumnades benefits treating rheumatoid arthritis and rheumatic arthritis parvathi, S. & Brindha, Ethnobotanical..., primer dimers also generate fluorescence because of the mid dorsal scales strongly keeled: Zaocys of Materia ]... Liquors, or ground for external use make a special health drink at restaurants! Of growth or through Cao, M. et al Agkistrodon has a certain therapeutic effect on pathological... Specific Name: Zaocys or more registered doctors before take any actions bile from fluorescence! Ptyas dhumnades misidentifying the origin of the Z. dhumnades and counterfeits 9 zaocys dhumnades benefits 12 g. Clipboard, Search History and. Cantor ) dye in double-stranded DNA recommendation engine FRET ratio of no-template control, PCR product of adulterant.... T. Inflammation in.gov or.mil Mohsin, M. & Ahmad, a..... Considered to be healthier and safer than synthetic medicines2 and to clear eye! Yellowish white or pale brown, ribs neatly arrange Generally, tan or black Wu Shao She with slightly. These amateur observations of Ptyas dhumnades plains, hills, and eczema respectively ribs neatly arrange
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